Quantitation of beta-actin-specific mRNA transcripts using xeno-competitive PCR.
نویسندگان
چکیده
منابع مشابه
Absolute quantitation of MDR1 transcripts using heterologous DNA standards--validation of the competitive RT-PCR (CRT-PCR) approach.
The multidrug resistance (MDR1) gene product, P-glycoprotein (Pgp), is a 170-kDa ATP-dependent pump that expels a variety of anticancer drugs out of malignant cells, reducing drug accumulation and thus antitumor activity. In recent years, considerable data has been presented that indicates the need to standardize detection methods for Pgp and MDR1. Reverse transcription (RT)-PCR is one of the m...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1993
ISSN: 1088-9051
DOI: 10.1101/gr.3.1.57